EFFECT OF MAGNESIUM SUPLEMENTATION...

EFFECT OF MAGNESIUM SUPLEMENTATION ON OXIDATIVE STRESS IN ALLOXANIC DIABETIC RATS.

Revista Magnesium Reserch – volume 16 – ano 2003 Número 1; páginas 13- 19.
 

Autores:

1) Chetan P. Hans – Department of biochemistry, Panjab University, Chandijgarth, India 160014.

2) Dharam P. Chaudhary - Department of biochemistry, Panjab University, Chandijgarth, India 160014.

3) Devi D. Bansal  - Department of biochemistry, Panjab University, Chandijgarth, India 160014.


Summary:


Magnesium deficit and oxidative stress are common features of the diabetics state. This concept supported by another observation that magnesium deficiency is also a state of increased oxidative stress prompted us to study the effect of magnesium supplementation on magnesium status and oxidative stress in diabetic rats. For this purpose, male Wistar rats were made diabetic with a single intraperitoneal injection of Alloxan. Experimental diabetes caused a significant decreased in serum and red blood cell magnesium levels and increased urinary excretion of magnesium. Marked increased in plasma malondialdehyde and corresponding decrease in vitamins C&E, uric acid and total thiols was observed in the diabetic rats as compared to control group. in liver, MDA levels were increased significantly with concomitant decrease in vitamin c, nonprotein thiols and antioxidant enzymes (SOD & GST). Magnesium supplementation for four weeks restored serum and RBC magnesium levels to near normal levels with marginal but significant decrease in blood glucose levels. Plasma and liver MDA levels were reduced significantly and vitamin C and total thiols were increased significantly with magnesium supplementation. Antioxidant enzyme activity was also increased significantly with magnesium supplementation in diabetics rats. Our data clearly demonstrates that alloxanic diabetes is associated with decreased magnesium status and increased oxidative stress and that magnesium supplementation can in part restore the antioxidant parameters and decrease the oxidative stress in experimental diabetic rats.